Journal: Translational Oncology

Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response

doi: 10.1016/j.tranon.2025.102428

Figure Lengend Snippet: IL1RA is associated with oncogenic signaling pathways and type I interferon response in OSCC in vitro . A. Volcano plots visualizing 132 DEGs between CAL27 cells transfected with NC and oeIL1RA. Down-regulated, up-regulated and non-regulated genes were labeled in blue, red, and grey colors, respectively. B. GSEA showed less enriched cancer-related features or processes in CAL27 cells overexpressing IL1RA. C. GSEA showed that IL1RA overexpression was significantly associated with the type I interferon response. D. A Venn diagram visualizing 45 (61.6 %) OSCC patients simultaneously carrying mutations in the IL1RA, IFNA , and IFNB genes in the TCGA dataset.

Article Snippet: Slices were then incubated with diluted primary antibodies against IL1RA (NBP1-32568, Novusbio, 1:600), E-Cadherin (#13116, CST, 1:400), N-Cadherin (#3195, CST, 1:100), Vimentin (#5741, CST, 1:100), IFNA (18013-AP, Proteintech, 1:400), and IFNB (27506-AP, Proteintech, 1:400) overnight at 4° C, and the secondary antibody (horseradish peroxidase-labeled goat anti-rabbit) at room temperature for 2 h. Cell nuclei were dyed with a DAB staining solution.

Techniques: Protein-Protein interactions, In Vitro, Transfection, Labeling, Over Expression

Journal: Translational Oncology

Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response

doi: 10.1016/j.tranon.2025.102428

Figure Lengend Snippet: Overexpression of IL1RA up-regulates type I interferon proteins in OSCC in vitro . A. H&E (the first lane) and IHC staining (the latter three lanes) of IFNA and IFNB in OSCC specimens of the high and low IL1RA expression groups (n=30, scale bar=100 μm). B-C. The mRNA (B) and protein expressions (C) of IFNA and IFNB in OSCC specimens of the high and low IL1RA expression groups. * P <0.05, and ** P <0.01 vs. NC group.

Article Snippet: Slices were then incubated with diluted primary antibodies against IL1RA (NBP1-32568, Novusbio, 1:600), E-Cadherin (#13116, CST, 1:400), N-Cadherin (#3195, CST, 1:100), Vimentin (#5741, CST, 1:100), IFNA (18013-AP, Proteintech, 1:400), and IFNB (27506-AP, Proteintech, 1:400) overnight at 4° C, and the secondary antibody (horseradish peroxidase-labeled goat anti-rabbit) at room temperature for 2 h. Cell nuclei were dyed with a DAB staining solution.

Techniques: Over Expression, In Vitro, Immunohistochemistry, Expressing

Journal: Translational Oncology

Article Title: IL1RA inhibits the progression of oral squamous cell carcinoma by mediating type Ⅰ interferon response

doi: 10.1016/j.tranon.2025.102428

Figure Lengend Snippet: IL1RA promotes the expressions of IFNA and IFNB in the OSCC xenografts and their release in OSCC cells. A. IHC staining of positive expressions of IFNA and IFNB in OSCC xenografts of oeIL1RA group and NC group in vivo (scale bar=100 μm). B. The mRNA levels of IFNA and IFNB in OSCC xenografts of the oeIL1RA group and NC group in vivo . C. ELISA showed contents of IFNA and IFNB in the cell supernatant of the oeIL1RA group and NC group in vitro . * P <0.05, and ** P <0.01 vs. NC group.

Article Snippet: Slices were then incubated with diluted primary antibodies against IL1RA (NBP1-32568, Novusbio, 1:600), E-Cadherin (#13116, CST, 1:400), N-Cadherin (#3195, CST, 1:100), Vimentin (#5741, CST, 1:100), IFNA (18013-AP, Proteintech, 1:400), and IFNB (27506-AP, Proteintech, 1:400) overnight at 4° C, and the secondary antibody (horseradish peroxidase-labeled goat anti-rabbit) at room temperature for 2 h. Cell nuclei were dyed with a DAB staining solution.

Techniques: Immunohistochemistry, In Vivo, Enzyme-linked Immunosorbent Assay, In Vitro

Journal: Emerging microbes & infections

Article Title: Novel transcription and replication-competent virus-like particles system modelling the Nipah virus life cycle.

doi: 10.1080/22221751.2024.2368217

Figure Lengend Snippet: Figure 6. Utility of trVLP-NiV in evaluating the effects of different kinds of antiviral agents. (A) 293T-NPL cells were infected with trVLP-NiV and cultured with 10 μM of FEMA 4809, remdesivir, and ribavirin, respectively. DMSO was used as a control. After 24 h infection, RLuc activity was quantified and normalized to DMSO-treated cells. Antiviral effect of ribavirin (B), remdesivir (C), IFN-α (D), and IFN-β (E). The 293T-NPL cells were infected with trVLP-NiV and treated with indicated concentrations of ribavirin or remdesivir. For IFN-α and IFN-β, cells were pre-treated with IFN-α or IFN-β for 8 h before infection. After 24 h of infection, RLuc activity (dark orange) and cell viability (blue) were measured. (F to H) Evaluating neutralizing activity with trVLP-NiV. The indicated concentrations of mAbs were incubated with trVLP-NiV for 1 h at 37 °C. In addition, residual infectivity was determined using 293T-NPL cells. IC50, the half-maximum inhibitory concentration; CC50, 50% cytotoxic concentration. N = 3. Error bars indi cate SEM. NS, no significance; *P < 0.05; **P < 0.01. Significance assessed by student’s t-test.

Article Snippet: For interferon-α (IFN-α) and IFN-β treatment, the cells were pre-treated with different concentrations of IFN-α (MCE, HYP73246) and IFN-β (Sino Biological, 10704-HNAS-5) 8 h prior to infection.

Techniques: Infection, Cell Culture, Control, Activity Assay, Incubation, Concentration Assay